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KMID : 1177720110050010057
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Journal of Alternatives to Animal Experiments
2011 Volume.5 No. 1 p.57 ~ p.60
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Evaluation of moisturizing activity of Lipid-coated powder using a reconstructed human epidermis
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Jang Won-Hee
Jung Kyoung-Mi
Choi Sun-Kyung
Jung Haeng-Sun
Lee Su-Hyon
Choi Yeong-Jin
Park Young-Ho
Lim Kyung-Min
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Abstract
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Dry skin is induced by excessive transdermal water loss due to the damages of intracellular lipids. Moisturizer is safe and effective for improving dry skin, however, there is few efficient alternative method to evaluate moisturizing activity of new cosmetic ingredients. Here, we investigated the moisturizing activity of a face powder coated with mannosylerithritol lipids (MEL) that are similar to ceramides in structure and intermediates in the biosynthesis of intracellular lipids, without using animals or human but Keraskin™, a three-dimensional reconstructed human epidermis. After Keraskin™ was incubated, MEL-coated sericite or uncoated sericite was applied and then sodium lauryl sulphate (SLS) was treated with a nylon mesh covering to spread it uniformly. After incubation for 15 min, Keraskin™ was washed and it was post-incubated for 24 hr. At the end of post-incubation, Keraskin™ was incubated with MTT for 3 hr to measure viability. Formed purple formazan was extracted with isopropanol for 2 hr. Absorbance of extract(A590) was measured using spectrophotometer. Cell viability was calculated by dividing with the absorbance of negative control. The viability of reconstructed epidermis treated 10% SLS was determined to be 12.3 ± 4.87%. When SLS was co-treated with sericite or MEL-coated sericite, cell viability was increased to 23.7 ± 2.92% or 71.5 ± 20% (p<0.05), respectively. MEL-coated sericite inhibited SLS-induced cytotoxicity substantially in comparison with sericite alone. Based on these results, reconstructed human epidermis can be used for the evaluation of the efficacy of cosmetic ingredients or products.
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KEYWORD
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reconstructed human epidermis, moisturizing activity, mannosylerithritol Lipid
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